Improved bioethanol production in an engineered Kluyveromyces lactis strain shifted from respiratory to fermentative metabolism by deletion of NDI1
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Improved bioethanol production in an engineered Kluyveromyces lactis strain shifted from respiratory to fermentative metabolism by deletion of NDI1
학술지
Microbial biotechnology
저자명
Gonzá lez-Siso, Marí a Isabel; Touriñ o, Alba; Vizoso, Á ngel; Pereira-Rodrí guez, Á ngel; Rodrí guez-Belmonte, Esther; Becerra, Manuel; Cerdá n, Marí a Esperanza
초록
<P>In this paper, we report the metabolic engineering of the respiratory yeast <I>K</I><I>luyveromyces lactis</I> by construction and characterization of a null mutant (<I>Δ</I><I>klndi</I><I>1)</I> in the single gene encoding a mitochondrial alternative internal dehydrogenase. Isolated mitochondria of the <I>Δ</I><I>klndi</I><I>1</I> mutant show unaffected rate of oxidation of exogenous NADH, but no oxidation of matrix NADH; this confirms that <I>Kl</I>Ndi1p is the only internal NADH dehydrogenase in <I>K</I><I>. lactis</I> mitochondria. Permeabilized cells of the <I>Δ</I><I>klndi</I><I>1</I> mutant do not show oxidation of matrix NADH, which suggests that shuttle systems to transfer the NADH from mitochondrial matrix to cytosol, for being oxidized by external dehydrogenases, are not functional. The <I>Δ</I><I>klndi</I><I>1</I> mutation decreases the chronological life span in absence of nutrients. The expression of <I>KlNDI</I><I>1</I> is increased by glutathione reductase depletion. The <I>Δ</I><I>klndi</I><I>1</I> mutation shifts the <I>K</I><I>. lactis</I> metabolism from respiratory to fermentative: the <I>Δ</I><I>klndi</I><I>1</I> strain shows reduced respiration rate and increased ethanol production from glucose, while it does not grow in non-fermentable carbon sources such as lactate. The biotechnological benefit of the <I>Δ</I><I>klndi</I><I>1</I> mutant for bioethanol production from waste cheese whey lactose was proved.</P>