BioChem - DOS

High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: A comparison between SPORL and dilute acid pretreatments

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Zhu, J.Y.; Gleisner, R.; Scott, C.T.; Luo, X.L.; Tian, S.

<P><B>Highlights</B></P><P>&#x025BA; High solids SSF using aspen at 18% solids with10FPU cellulase/g glucan. &#x025BA; SPORL pretreatment produced ethanol yield 211L/ton wood at 59g/L. &#x025BA; Dilute acid pretreatment produced 133L/ton wood at 35g/L.</P> <P><B>Abstract</B></P><P>Native aspen (<I>Populus tremuloides</I>) was pretreated using sulfuric acid and sodium bisulfite (SPORL) and dilute sulfuric acid alone (DA). Simultaneous enzymatic saccharification and fermentation (SSF) was conducted at 18% solids using commercial enzymes with cellulase loadings ranging from 6 to 15FPU/g glucan and <I>Saccharomyces cerevisiae</I> Y5. Compared with DA pretreatment, the SPORL pretreatment reduced the energy required for wood chip size-reduction, and reduced mixing energy of the resultant substrate for solid liquefaction. Approximately 60% more ethanol was produced from the solid SPORL substrate (211L/ton wood at 59g/L with SSF efficiency of 76%) than from the solid DA substrate (133L/ton wood at 35g/L with SSF efficiency 47%) at a cellulase loading of 10FPU/g glucan after 120h. When the cellulase loading was increased to 15FPU/g glucan on the DA substrate, the ethanol yield still remained lower than the SPORL substrate at 10FPU/g glucan.</P>

2011

0960-8524

102

19

pp.8921-8929

10.1016/j.biortech.2011.07.047

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57231278

(Woody) Biomass; Pretreatment; High solids; Simultaneous saccharification and fermentation (SSF); Cellulosic ethanol titer