Barragá n-Trinidad, Martí n; Carrillo-Reyes, Juliá n; Buitró n, Germá n
초록
<P><B>Abstract</B></P> <P>The use of ruminal fluid as a source of hydrolytic microorganisms for the pretreatment of a native consortium of microalgae (essentially <I>Senedesmus</I>) was investigated. The hydrolytic enzyme activity of the ruminal culture was first enriched in a bioreactor. Then, using the enriched culture, the effect of the microalgae to the ruminal fluid ratio (S/X) on the hydrolysis and subsequent production of methane was investigated. An S/X ratio of 0.5 showed the best hydrolysis efficiency (29%) reaching in a second stage process a methane yield of 193mL CH<SUB>4</SUB> g COD<SUP>−1</SUP>. The processing time (pretreatment plus methanization) was only 7days. The predominant ruminal hydrolytic bacteria selected in the enrichment were principally <I>Clostridium</I>, <I>Proteocatella</I> and <I>Pseudomonas</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microalgal cell disruption by ruminal microorganisms was studied. </LI> <LI> An enrichment of microorganisms solubilizing algal biomass was conducted. </LI> <LI> Microalgae to ruminal fluid ratio influences the hydrolysis and methane production. </LI> <LI> The microalgae hydrolysis and methane production were obtained in only 7days. </LI> </UL> </P>