초록
<P><B>Abstract</B></P> <P>Co-culture is a new strategy to enhance metabolite biosynthesis of plants. In the present study, effects of Murashige and Skoog (MS) medium strengths and sucrose concentrations on adventitious root (AR) biomass, bioactive compound accumulation, and antioxidant activity were investigated to optimize the bioreactor co-culture conditions of the ARs of <I>Echinacea pallida</I> and <I>Echinacea purpurea</I>. A kinetic study was also conducted to confirm the suitable culture period. MS medium strengths and sucrose concentrations critically affected the efficiency of the AR co-culture. The maximum AR biomass and accumulation of total polysaccharide, total phenolics, total caffeic acid derivatives, and their monomers (caftaric acid, chlorogenic acid, caffeic acid, cynarine, echinacoside, and cichoric acid) were determined at 0.75× MS medium supplemented with 50 g L<SUP>−1</SUP> sucrose, resulting in the occurrence of the highest antioxidant activities, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power. In the kinetic study, the bioactive compound production peaked on day 35, and 502.1 mg L<SUP>−1</SUP> polysaccharides, 564.3 mg L<SUP>−1</SUP> phenolics, 457.4 mg L<SUP>−1</SUP> flavonoids, and 416.1 mg L<SUP>−1</SUP> caffeic acid derivatives were produced. The DPPH radical scavenging activity and reducing power also reached the maximum on day 35 of culturing. Therefore, the optimized conditions of the AR co-culture of <I>E. pallida</I> and <I>E. purpurea</I> can be used for the mass production of bioactive compounds during the industrial production of <I>Echinacea</I> products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Adventitious roots (ARs) of two <I>Echinacea</I> species were co-cultured in a bioreactor. </LI> <LI> Culture conditions of AR co-culture in the bioreactor were optimized. </LI> <LI> 0.75 × MS medium supplemented with 50 g L<SUP>−1</SUP> sucrose was the most favorable. </LI> <LI> The suitable culture period for AR co-culture was 35 days. </LI> </UL> </P>