BioChem - DOS

CO2 sequestration potential of halo-tolerant bacterium Pseudomonas aeruginosa SSL-4 and its application for recovery of fatty alcohols

Process safety and environmental protection : transactions of the Institution of Chemical Engineers, Part B

Mishra, Somesh; Chauhan, Piyush; Gupta, Suresh; Raghuvanshi, Smita; Singh, Rajnish P.; Jha, Prabhat N.

<P><B>Abstract</B></P> <P>Bio-mitigation of CO<SUB>2</SUB> utilizing prokaryotes and simultaneous extraction of valuable bio-molecule is fast gaining interest now-a-days. Present work discusses the thermodynamic assessment of CO<SUB>2</SUB> bio-mitigation capability of <I>Pseudomonas aeruginosa</I> SSL-4 isolated from halo alkalophilic habitat in the absence of light. The maximum specific growth (<I>&mu;</I> <SUB>Max</SUB>, h<SUP>&minus;1</SUP>) of isolate was found to be 0.425 (&plusmn;0.0025) and 0.34 (&plusmn;0.0063) at 3% (w/v) salt concentration and 35&deg;C, respectively. The isolate was cultivated in the environment having initial CO<SUB>2</SUB> (g) concentration of 17(&plusmn;0.8) % (v/v) using Fe[II] as an energy source (0, 50 and 100ppm) for evaluating CO<SUB>2</SUB> fixing ability of microorganisms. The maximum CO<SUB>2</SUB> removal efficiency of 92.37 (&plusmn;2.46) % (v/v) was obtained at 100ppm of Fe[II] concentration. The isolate has shown the maximum CO<SUB>2</SUB> fixation rate ( <SUB> R <SUB> C O 2 </SUB> </SUB> ) of 0.04 (&plusmn;0.003) and 0.06 (&plusmn;0.001) g/L/d at 50 and 100ppm of Fe[II] concentration, respectively. FT-IR and GC&ndash;MS analysis of obtained leachate revealed the presence of fatty alcohols (C<SUB>12</SUB>&ndash;C<SUB>28</SUB>) and total product recovery (C<SUB>12</SUB>&ndash;C<SUB>18</SUB>) of 0.371g per g of biomass. The thermodynamic assessment revealed the actual CO<SUB>2</SUB> utilization efficiency of 41.16%. Thus, the isolated strain from extreme hyper saline environment has shown the potential for research dedicated to carbon capture and utilization.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bio-mitigation of CO<SUB>2</SUB> utilizing bacterium isolated from extreme haloalkalophilic habitat. </LI> <LI> Bacterium utilizes Iron as an energy source for CO<SUB>2</SUB> fixation. </LI> <LI> Thermodynamic assessment for CO<SUB>2</SUB> fixation by isolated bacterium was carried out. </LI> <LI> Call lysate extract of <I>Pseudomonas aeruginosa</I> SSL-4 shows the presence of fatty alcohols and hydrocarbons. </LI> <LI> Material balance and thermodynamic evaluation of the batch studies confirms the assimilation of CO<SUB>2</SUB> as biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2017

Elsevier

0957-5820

111

pp.582-591

10.1016/j.psep.2017.08.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78804778

CO2 mitigation; Thermodynamic assessment; Energy source; FT-IR; GC&#x2013; MS; Fatty alcohol;