BioChem - DOS

Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization

Journal of agricultural and food chemistry

Bao, Ruiqi; Gao, Ning; Lv, Jing; Ji, Chaofan; Liang, Huipeng; Li, Shengjie; Yu, Chenxu; Wang, Zhenyu; Lin, Xinping

<P>Nine transformants of <I>Rhodosporidium toruloides</I> with significant changes in the carotenoid profile were obtained by <I>Agrobacterium tumefaciens</I>-mediated transformation, including a white, three red, and four yellow mutants. A red mutant A1-15-BRQ that showed a high torularhodin production was selected for culture condition optimization. Results indicated that the torularhodin yield was boosted with glucose as the carbon source, at a carbon/nitrogen ratio of 22, a loading volume of 75 mL, and 28 °C. The torularhodin yield of 21.3 mg/L consisting of 94.4% total carotenoids was obtained by Box-Behnken design experiments. The torularhodin yield was 17.0-fold higher than that of the wild type, with time shortened from 9 to 3 days. This study reports an effective strategy for improving torularhodin production and provides a candidate <I>R. toruloides</I> strain for highly selective production of torularhodin.</P><BR>[FIG OMISSION]</BR>

2019

American Chemical Society

0021-8561

1520-5118

67

4

pp.1156-1164

10.1021/acs.jafc.8b04667.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART95596935

https://doi.org/10.1021/acs.jafc.8b04667.s001

Rhodosporidium toruloides; torularhodin; Agrobacterium tumefaciens-mediated transformation; response surface methodology; selective production