초록
This work adopted an innovative approach for fermentative H<SUB>2</SUB> production from common domestic organic waste, at 28 <SUP>o</SUP>C, in the absence of pretreatment: the self-fermentation of non-sterile vegetable waste and the bioaugmentation of microbial indigenous fermenting communities. For this purpose, three new H<SUB>2</SUB>-producing strains, Buttiauxella sp. 4, Rahnella sp. 10 and Raoultella sp. 47, isolated and enriched from vegetable waste, were individually tested on two types of vegetable waste and compared with a bacterial artificial consortium composed of the three strains put together. The three single strains were also characterized for their ability to produce H<SUB>2</SUB> on different sugars, such as xylose, arabinose and cellobiose, as these are key products of hydrolysis of cellulose and hemicelluloses. H<SUB>2</SUB> production occurred from self-fermentation with yields ranging from 18.08 to 21.95 ml H<SUB>2</SUB>/g VS. All bacterial inocula promoted a significant increase of the H<SUB>2</SUB> yield and the H<SUB>2</SUB> production rate, compared to the self-fermentation. The inocula of the artificial consortium yielded the maximum H<SUB>2</SUB> production of 85.65 ml H<SUB>2</SUB>/g VS with the highest H<SUB>2</SUB> production rate of 2.56 ml H<SUB>2</SUB>/h.