BioChem - DOS

Isolation of Candida rugosa lipase isoforms

Hemijska industrija

Trbojevic N., Jovana; Dimitrijevic S., Aleksandra; Velickovic V., Dusan; Gavrovic-Jankulovic, Marija; Milosavic B., Nenad

<P>Convenient source of lipases for science and industry is yeast Candida rugosa. Crude preparation of Candida rugosa lipase (CRL) consists of several extracellular lipases. Isoenzyme profile depends on the culture or fermentation conditions. All isoforms are coded by lip pseudogene family; they are monomers of 534 amino acids and molecular weight of about 60 kDa. They share the same catalytic mechanism and interfacial mode of activation. Isoenzymes differ in isoelectric points, post-translational modifications, substrate specificity and hydrophobicity. The presence of different lipase isoforms and other substances (i. e. inhibitors) in crude preparation leads to lack of their productivity in biocatalytic reactions. Purification of specific isoform improves its overall performance and stability. This paper provides an overview of different methods for purification of CRL isoenzymes up to date, their advantages and disadvantages.</P>

2013

National Library of Serbia

0367-598x

2217-7426

67

5

pp.703-706

10.2298/hemind120828113t

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART92834300

http://www.doiserbia.nb.rs/ft.aspx?id=0367-598X1200113T