초록
<P>The continuous biocatalytic synthesis of (<I>R</I>)-2-octanol carried out in an enzyme membrane reactor showed an excellent process stability of the biocatalysts, resulting in a turnover number of 15 million for the applied alcohol dehydrogenase. Utilisation of the ionic liquid AMMOENG™ 101 as a feasible cosolvent increased substrate concentration, and improved space time yields and turnover numbers of the cofactor by factors of 3 and 6, respectively. Moreover, 80% less waste was generated, while producing the same amount of product. Integrated product separation was realized <I>via</I> solid phase extraction. Extraction of the applied solid phase with supercritical carbon dioxide allowed more than 30 reuses of the solid phase.</P> <P>Graphic Abstract</P><P>The continuous biocatalytic synthesis of (<I>R</I>)-2-octanol carried out in an enzyme membrane reactor showed an excellent process stability of the biocatalysts, resulting in a turnover number of 15 million for the applied alcohol dehydrogenase.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0gc00790k'><BR></P>