초록
<P><B>Abstract</B></P> <P>Thermal acid hydrolysis is often used to deal with lignocellulosic biomasses, but 5-hydroxy-methylfurfural (5-HMF) formed during hydrolysis deeply influences downstream fermentation. 2,5-Furan-dicarboxylic acid (FDCA), which is in the list of future important biomass platform molecules can be obtained using 5-HMF biotransformation. Based on the connection between 5-HMF removal in acid hydrolysate and FDCA production, the optimum thermal acid hydrolysis condition for macroalgae <I>Chaetomorpha linum</I> was established. Potential microbes capable of transforming 5-HMF into FDCA were isolated and characterized under various parameters and inoculated into algal hydrolysate to perform 5-HMF biotransformation. The optimum hydrolysis condition was to apply 0.5M HCl to treat 3% algal biomass under 121°C for 15min. Isolated <I>Burkholderia cepacia</I> H-2 could transform 2000mg/L 5-HMF at the initial pH of 7 at 28°C and 1276mg/L FDCA was received. Strain <I>B</I>. <I>cepacia</I> H-2 was suitable for treating the algal hydrolysate without dilution, receiving 989.5mg/L FDCA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Optimum thermal acid hydrolysis condition of <I>Chaetomorpha linum</I> was established. </LI> <LI> Strain <I>Burkholderia cepacia</I> H-2 biotransforming 5-HMF into FDCA was isolated. </LI> <LI> 5-HMF concentration and pH value deeply influenced FDCA production. </LI> <LI> 5-HMF biotransformation in the acid algal hydrolysate was performed. </LI> <LI> 5-HMF detoxification and FDCA production in the algal hydrolysate was feasible. </LI> </UL> </P>