초록
<P><B>Abstract</B></P> <P>This study evaluated the effect of attapulgite addition on the anaerobic digestion of pig slurry. Results showed that attapulgite addition increased methane yield by 8.9%–37.3% and affected methane production kinetics. Attapulgite with the addition loading of 10 g/L obtained the highest methane yield of 210.4 mL/g volatile solids. Attapulgite accelerated the rates of hydrolysis, acetogenesis, and methanogenesis, as evidenced by the increases in the activities of <I>β</I>-glucosidase, protease, dehydrogenase, and coenzyme F<SUB>420</SUB>; the abundance of hydrolytic and acetogenic bacteria (Clostridiales, Syntrophobacterales, and Fibrobacterales); and the abundance of methanogenic microorganisms (Methanomicrobiales). These phenomena can be ascribed to both the reduced ammonia nitrogen due to attapulgite adsorption and the increased trace elements, such as Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, K<SUP>+</SUP>, and Fe<SUP>3+</SUP> ions, due to attapulgite release. However, excessive dose of attapulgite (≥30 g/L) decreased the promotion of methane production by reducing the enzyme activities of hydrolytic and methanogenic steps and lowering the abundance of hydrolytic, acetogenic, and methanogenic microorganisms due to the high levels of free ammonia and metal ions, such as Mg<SUP>2+</SUP>, at the initial phase of digestion. These results provided insights into the improvement of methane production during the anaerobic digestion of animal slurry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The effect of attapulgite on the process performance of pig slurry digestion was evaluated. </LI> <LI> Attapulgite addition increased methane yield by 8.9%–37.3%. </LI> <LI> Attapulgite improved the <I>β</I>-glucosidase, protease, dehydrogenase and F<SUB>420</SUB> activities. </LI> <LI> Attapulgite increased the hydrolytic, acetogenic and methanogenic microorganisms. </LI> <LI> Attapulgite adsorbed ammonia nitrogen and released Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, K<SUP>+</SUP> and Fe<SUP>3+</SUP> ions. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>