초록
The aim of this study was to promote biohydrogen production in an thermophilic anaerobic fluidized bed reactor (AFBR) at 55 <SUP>o</SUP>C using a mixture of sugar cane stillage and glucose at approximately 5000-5300 mg COD L<SUP>-1</SUP>. During a reduction in the hydraulic retention time (HRT) from 8, 6, 4, 2 and 1 h, H<SUB>2</SUB> yields of 5.73 mmol g COD<SUB>added</SUB><SUP>-1</SUP> were achieved (at HRT of 4 h, with organic loading rate of 52.7 kg COD m<SUP>-3</SUP> d<SUP>-1</SUP>). The maximum volumetric H<SUB>2</SUB> production of 0.78 L H<SUB>2</SUB> h<SUP>-1</SUP> L<SUP>-1</SUP> was achieved using stillage as carbon source. In all operational phases, the H<SUB>2</SUB> average content in the biogas was between 31.4 and 52.0%. Butyric fermentation was the predominant metabolic pathway. The microbial community in accordance with the DGGE bands profile was found similarity coefficient between 91 and 95% without significant changes in bacterial populations after co-substrate removal. Bacteria like Thermoanaerobacterium sp. and Clostridium sp. were identified.