초록
<P><B>Abstract</B></P> <P>The hydrogen over-producing <I>Escherichia coli</I> HD701, a hydrogenase up-regulated strain, has the potential for industrial-scale hydrogen production; however, this strain is unable to metabolize sucrose which is a major constituent of many waste organic materials that can be used as feedstock for industrial hydrogen production. Invertase from <I>Sacharomyces cervacea</I> (yeast) was partially purified and characterized where its apparent optimum temperature when using short reaction period (15 min) was 55 °C; however the enzyme couldn't continue active due to its short half-life time at such high temperature. In contrast, a lower optimum temperature (35 °C) was recorded when using long reaction period (5 h) where the enzyme showed long half-life time and stability at such degree of temperature. Consequently, a concomitant hydrolysis of sucrose by yeast invertase and hydrogen production by <I>E. coli</I> at 35 °C was conducted and showed a high potency for industrial application with a hydrogen yield of 0.48 mole hydrogen/mole reducing sugars using batch fermentation at optimum sucrose concentration of 10 g/L. The described approach might be applicable for biotechnologies of other bio-products by <I>E. coli</I> from sucrose as a carbon source.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Concomitant hydrolysis of sucrose by yeast invertase and H<SUB>2</SUB> production by <I>E. coli</I> was conducted. </LI> <LI> Invertase of yeast showed long half-life time with high stability at 35 °C. </LI> <LI> Yeast invertase is highly stable for hydrolysis of sucrose concomitantly with H<SUB>2</SUB> production by <I>E. coli.</I> </LI> <LI> The described approach might be applicable for biotechnologies of other bio-products. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>