BioChem - DOS

On the use of resting L. delbrueckii spp. delbrueckii cells for D-lactic acid production from orange peel wastes hydrolysates

Biochemical engineering journal

de la Torre, I.; Acedos, M.G.; Ladero, M.; Santos, V.E.

<P><B>Abstract</B></P> <P>The availability of pure isomers of lactic acid is a key aspect of the fabrication of polymers based on poly-lactic acid with enhanced mechanical and thermal properties. The main problems presented in the fermentation process to <SMALL>D</SMALL>-Lactic acid production are the costs derived from the carbon and nitrogen sources and the purification ones. In this work, the production is carried out using hydrolyzed orange peel wastes (OPW) as a cheap carbon source and a resting cells production process is developed to avoid the nitrogen source costs and to reduce them in the purification processes. A strategy to adapt the strain for the uptake of the mixture of sugars presented in the orange peel hydrolysate is developed in order to improve the fructose and galactose uptake rates. A <SMALL>D</SMALL>-lactic acid productivity important increase (6.72 g/L&middot;h) is obtained from OPW hydrolysates using resting <I>L. delbrueckii</I> cells when grown on fructose during the growth stage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new strategy is proposed for cells production in order to optimize the sugars uptake. </LI> <LI> The adaptation of the cells to the mixture of sugars from the orange peel waste is mandatory. </LI> <LI> The use of resting cells improves the results for <SMALL>D</SMALL>-lactic acid production. </LI> <LI> 6.72 g/L.h (productivity) and 87.8% (yield) are achieved from orange peel wastes hydrolysates. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2019

Elsevier

1369-703x

145

pp.162-169

10.1016/j.bej.2019.02.012

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART95829666

Resting cells; D-lactic acid; Orange peel waste hydrolysates; Lactobacillus delbrueckii