BioChem - DOS

Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Xia, L.; Ge, H.; Zhou, X.; Zhang, D.; Hu, C.

In this study, Scenedesmus obtusus XJ-15 was firstly selected from seven strains microalgae (Chlorophyta, Scenedesmaceae) and then cultivated using a two-stage strategy, which composed of fast cell growth in stage I and followed by lipid induction in stage II in 5-L flasks outdoors. In stage I, the biomass productivity was increased from 139.4 to 212.1mgL<SUP>-1</SUP>d<SUP>-1</SUP>. In stage II, lipid content was increased from 26.1% to 47.7% by adding NaCl into the culture. This two-stage process was also realized in an 140-L photobioreactor outdoors, with a biomass productivity of 86.5mgL<SUP>-1</SUP>d<SUP>-1</SUP> and CO<SUB>2</SUB> fixation rate of 170.0mgL<SUP>-1</SUP>d<SUP>-1</SUP> in the first stage, and high lipid content of 42.1% in the second stage. With such a culture strategy, the overall lipid productivity was improved and better biodiesel quality was obtained. These results suggested the photoautotrophic two-stage system was not only feasible but also effective.

2013

Elsevier Applied Science

0960-8524

144

pp.261-267

10.1016/j.biortech.2013.06.112

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66561861

Scenedesmus obtusus XJ-15; Biodiesel; Outdoors; Photoautotrophic two-stage cultivation; Lipid induction