초록
<P><B>Abstract</B></P> <P>Indiscriminate disposal of the solid waste generated from various agricultural practices cause detrimental effects on the environment. Utilization of the waste biomass for production of value-added products through biotechnological intervention not only helps to combat environmental pollution but also contributes significantly to the economy. In this study, a <I>biofabrication software (fermentor tool)</I> was applied to analyze statistics of xylanase production under solid non-sterile fermentation conditions. In solid substrate fermentation wheat bran, banana peel, millet waste, and sugarcane bagasse were evaluated. Maximal xylanase titer of 10545 U/g dry matter was obtained when wheat bran was used as a substrate (4 days incubation at 50 °C and pH value of 8.0). Partially purified xylanase was applied in saccharification of lignocellulosic materials to assess its potential application in a lignocellulosic biorefinery. In this context, the precision of predicted mathematical model was evaluated and fermentation provided a good prediction of the concentration of cell, substrate and product. Second, open non-sterilized fermentation method was established to save sterilization cost that seems to be a very economical and practically applicable to the enzyme industry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Thermoalkalophilic strain with high xylanase production rate was obtained. </LI> <LI> Open non-sterile fermentation for xylanase production was developed. </LI> <LI> Agricultural solid waste was utilized for fermentable sugar production. </LI> <LI> Xylanase proved potential for saccharification of various lignocellulosic biomass. </LI> <LI> Biofabrication software was applied for mathematical model-based optimization. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>