Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production

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논문

Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production

학술지

Microbial cell factories

저자명

Anfelt, Josefine; Kaczmarzyk, Danuta; Shabestary, Kiyan; Renberg, Bjö rn; Rockberg, Johan; Nielsen, Jens; Uhlé n, Mathias; Hudson, Elton P.

초록

BackgroundThere is a strong interest in using photosynthetic cyanobacteria as production hosts for biofuels and chemicals. Recent work has shown the benefit of pathway engineering, enzyme tolerance, and co-factor usage for improving yields of fermentation products.ResultsAn n-butanol pathway was inserted into a Synechocystis mutant deficient in polyhydroxybutyrate synthesis. We found that nitrogen starvation increased specific butanol productivity up to threefold, but cessation of cell growth limited total n-butanol titers. Metabolite profiling showed that acetyl-CoA increased twofold during nitrogen starvation. Introduction of a phosphoketolase increased acetyl-CoA levels sixfold at nitrogen replete conditions and increased butanol titers from 22 to 37 mg/L at day 8. Flux balance analysis of photoautotrophic metabolism showed that a Calvin–Benson–Bassham-Phosphoketolase pathway had higher theoretical butanol productivity than CBB-Embden–Meyerhof–Parnas and a reduced butanol ATP demand.ConclusionThese results demonstrate that phosphoketolase overexpression and modulation of nitrogen levels are two attractive routes toward increased production of acetyl-CoA derived products in cyanobacteria and could be implemented with complementary metabolic engineering strategies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-015-0355-9) contains supplementary material, which is available to authorized users.

발행연도

2015

발행기관

BioMed Central

라이선스

cc-by

ISSN

1475-2859

권

페이지

pp.167

DOI