초록
In this study, the composition and diversity of the bacterial community in thermophilic H<SUB>2</SUB>-producing reactors fed with glucose were investigated using pyrosequencing. The H<SUB>2</SUB>-producing experiments in batch were conducted using 0.5 and 2.0gl<SUP>-1</SUP> glucose at 550 <SUP>o</SUP>C. Under the two conditions, the H<SUB>2</SUB> production and yield were 1.3 and 1.6mol H<SUB>2</SUB>mol glucose<SUP>-1</SUP>, respectively. Acetic, butyric, iso-butyric, lactic and propionic acids were detected in the two reactors. The increase in substrate concentration favored a high H<SUB>2</SUB> yield. In this reactor, a predominance of acetic and iso-butyric acids, 27.7% and 40%, were measured, respectively. By means of pyrosequencing, a total of 323 and 247 operational taxonomic units were obtained, with a predominance of the phylum Firmicutes (68.73-67.61%) for reactors with 0.5 and 2.0gl<SUP>-1</SUP> glucose, respectively. Approximately 40.55% and 62.34% of sequences were affiliated with Thermoanaerobacterium and Thermohydrogenium, microorganisms that produce H<SUB>2</SUB> under thermophilic conditions.