BioChem - DOS

Biohydrogen production by vermihumus-associated microorganisms using agro industrial wastes as substrate

International journal of hydrogen energy

Oceguera-Contreras, Edé n; Aguilar-Juá rez, Oscar; Oseguera-Galindo, David; Mací as-Barragá n, José Bolañ os-Rosales, Roberto; Mena-Enrí quez, Mayra; Arias-Garcí a, Armando; Montoya-Buelna, Margarita; Graciano-Machuca, Omar; De Leó n-Rodrí guez, Antonio

<P><B>Abstract</B></P> <P>Typically dark fermentation is performed using activated sludge consortia and there are not studies on hydrogen production using vermihumus-associated microorganisms documented. Microorganisms from <I>Eisenia foetida</I> casting have proven an efficient anti-recalcitrant activity. We have evaluated hydrogen production by <I>Eisenia foetida</I> lixiviated earthworm as inoculum using agro industrial wastes as substrate. Experiments were performed in 120 ml serological bottles under anaerobic conditions at 40 &deg;C by triplicate. The results showed a hydrogen production of 1571.81, 1246.36 and 232.72 <SUB> ml H 2 </SUB> <SUP> L &minus; 1 </SUP> from molasses, bagasse and vinasse, respectively. Butyric and acetic acids were the main metabolites produced reaching up to 6.0 and 1.26 g <SUP> L &minus; 1 </SUP> , respectively. Butanol and ethanol were also produced, but the maximum concentrations attained were 1.7 and 0.28 g <SUP> L &minus; 1 </SUP> , respectively. Interestingly, the bagasse did not require a pretreatment. The results demonstrated that vermihumus-associated microorganisms are capable of producing hydrogen from the recalcitrant raw materials such as the sugarcane bagasse.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hydrogen production using lixiviated earthworm is a feasible methodology. </LI> <LI> Lixiviated earthworm is a cheap and nutrient rich feedstock. </LI> <LI> Pretreatment is not necessary to use the fermentable carbohydrates in wastes. </LI> <LI> Sugarcane molasses is the preferable substrate by this consortium. </LI> <LI> Vermihumus-associated microorganisms are capable of degrading sugarcane bagasse. </LI> </UL> </P>

2019

Elsevier

0360-3199

1879-3487

44

20

pp.9856-9865

10.1016/j.ijhydene.2018.10.236

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART96289087

Agro industrial wastes; Dark fermentation; Lignocellulosic; Vermihumus