초록
This study was conducted to evaluate the importance of aeration in free and immobilized cell systems in an aerated bioreactor for xylitol production from an oat hull hemicellulosic hydrolysate using an integrated process. The aeration rate (AR) or oxygen mass transfer coefficient (k<SUB>L</SUB>a) demonstrated a significant role in controlling cell (Candida guilliermondii FTI 20037) regeneration and bioconversion performance in free and immobilized cell systems. In the free cell system, an aeration rate of 1.25vvm corresponding to k<SUB>L</SUB>a of 15.81/h resulted in maximum values of product yield (Y<SUB>p/s</SUB>: 0.87g/g), productivity (Q<SUB>p</SUB>: 0.57g/l/h), and final xylitol concentration (P<SUB>f</SUB>: 55g/l) from the hydrolysate with a 74.5g/l xylose concentration. However, in the aerated immobilized cell system, maximum and almost similar results (almost P<SUB>f</SUB>: 54g/l, Q<SUB>p</SUB>: 0.57g/l/h and Y<SUB>p/s</SUB>: 0.84g/g) were obtained with aeration rates from 1.25 to 1.5vvm using composites based on polypropylene (PP) and partially delignified fiber (PDF). Composites based on acid treated fiber (ATF) containing a high amount of lignin showed some inhibitory impact on xylose uptake and xylitol formation (P<SUB>f</SUB>: 47g/l and Q<SUB>p</SUB><0.49g/l/h) with the optimal aeration rate of 1.5vvm in the initial cycle of the bioconversion; this inhibition impact could be resolved in the next consecutive cycles. The surface modifier polyethyleneimine (PEI) slightly enhanced cell retention in the immobilized form on the ATF-based cell support. This investigation helps fill in the knowledge gaps existing on the integrated processing of the lignocellulosic biomass for xylitol bioproduction and biorefinery industry; however, more scale-up studies are recommended for commercialization.