BioChem - DOS

Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cruz, M.V.; Paiva, A.; Lisboa, P.; Freitas, F.; Alves, V.D.; Simoes, P.; Barreiros, S.; Reis, M.A.M.

Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO<SUB>2</SUB>) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg<SUP>-1</SUP> CO<SUB>2</SUB>/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL<SUP>-1</SUP> with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL<SUP>-1</SUP>day<SUP>-1</SUP> and 0.77gg<SUP>-1</SUP>, respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34x10<SUP>5</SUP> and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.

2014

Elsevier Applied Science

0960-8524

157

pp.360-363

10.1016/j.biortech.2014.02.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69339780

Polyhydroxyalkanoates (PHAs); Cupriavidus necator; Spent coffee grounds (SCG); Triglycerides; Supercritical fluid extraction (SFE)