초록
<P><B>Abstract</B></P> <P>An integrated process enabling the simultaneous production and purification of fructo-oligosaccharides (FOS) was explored. A co-culture fermentation with <I>Aspergillus ibericus</I> (used as FOS producer strain) and <I>Saccharomyces cerevisiae</I> YIL162 W (for small saccharides removal) was optimized. Inoculation conditions of <I>S. cerevisiae</I>, fermentative broth composition, temperature and pH were optimized by experimental design. Yeast extract concentration and temperature were the most significant variables affecting FOS purity. Co-culture fermentations with simultaneously inoculation of the strains, run under 30 °C, initial pH 6.0 and 17 g L<SUP>–1</SUP> yeast extract led to FOS mixtures with 97.4 ± 0.2% (w/w) purity. The fermentations conducted in bioreactor, at a 0.8 vvm aeration rate, yielded 0.70 ± 0.00 g<SUB>FOS</SUB>.g<SUB>initial GF</SUB> <SUP>–1</SUP> at 45 h fermentation, with a FOS content of 133.7 ± 0.1 g L<SUP>−1</SUP>. A purity of FOS up to 93.8 ± 0.7% (w/w) was achieved. The one-step fermentation proved to be efficient, economical and fast.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Co-culture <I>Aspergillus ibericus</I>/<I>Saccharomyces cerevisiae</I> increases FOS yield/purity. </LI> <LI> Temperature, pH, yeast extract and aeration significantly affects FOS yield/purity. </LI> <LI> Simultaneous inoculation led to mixtures up to 97.4% of pure FOS. </LI> <LI> Bioreactor fermentations yielded 0.70 g<SUB>FOS</SUB>.g<SUB>in.GF</SUB> <SUP>–1</SUP> with 133.7 g L<SUP>−1</SUP> FOS content. </LI> <LI> One-step fermentation of 45 h lead to high-content FOS mixtures with high-purity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>