초록
<P><B>Abstract</B></P> <P>The DHA productivity of <I>Thraustochytrium</I> sp. BM2 was improved by optimizing the bioreactor operation conditions. The effects of agitation rate and aeration rate on the microalgal growth and DHA production were first studied in 5-L stirred-tank fermenter. The results showed that 100 rpm agitation and 1.2 vvm aeration (i.e., k<SUB>La</SUB> = 27 ± 1.1 h<SUP>−1</SUP>) achieved the highest biomass (4590 mg/L/d) and DHA (379 mg/L/d) productivities. Next, using an optimal glucose concentration (60 g/L) led to the highest biomass (5580 mg/L/d) and DHA (457 mg/L/d) productivities. The cellular DHA content decreased when increasing the agitation rate, probably due to the sensitivity of this strain to the shear stress. The effect of pneumatic and mechanical agitation under the same k<SUB>La</SUB> was studied with two different types of fermenters (stirred-tank and air-lift) and cultivation in air-lift fermenter resulted in better biomass production (27.57 g/L), cellular DHA content (13.1%) and DHA productivity (610 mg/L/d).</P> <P><B>Highlights</B></P> <P> <UL> <LI> An isolated <I>Thraustochytrium</I> sp. BM2 strain accumulates high DHA content. </LI> <LI> Higher dissolved oxygen in the medium promote DHA production of the BM2 strain. </LI> <LI> Air-lift bioreactor is more effective than stirred tank for DHA production with the BM2 strain. </LI> <LI> <I>Thraustochytrium</I> sp. BM2 demonstrates high potential as a microalgal DHA producer. </LI> </UL> </P>