BioChem - DOS

Evaluation of by-products from the biodiesel industry as fermentation feedstock for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Cupriavidus necator

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Garcia, I.L.; Lopez, J.A.; Dorado, M.P.; Kopsahelis, N.; Alexandri, M.; Papanikolaou, S.; Villar, M.A.; Koutinas, A.A.

Utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (PHA) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. Batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) with a 2.8-8% 3HV content. Fed-batch fermentations in shake flasks led to the production of 10.9g/L P(3HB-co-3HV) and a 55.6% P(3HB-co-3HV) content. NaCl concentrations between 2 and 6g/L gradually became inhibitory to bacterial growth and PHA formation, whereas in the case of K<SUB>2</SUB>SO<SUB>4</SUB>, the inhibitory effect was observed only at concentrations higher than 20g/L. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and nuclear magnetic resonance (<SUP>13</SUP>C NMR) demonstrated that the incorporation of 3HV into the obtained P(3HB-co-3HV) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. Integrating PHA production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.

2013

Elsevier Applied Science

0960-8524

130

pp.16-22

10.1016/j.biortech.2012.11.088

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65520639

Polyhydroxyalkanoates (PHA); Crude glycerol; Rapeseed meal hydrolysate; Microbial bioconversion; Cupriavidus necator