BioChem - DOS

Enhancement of n-butanol production by in situ butanol removal using permeating-heating-gas stripping in acetone-butanol-ethanol fermentation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Chen, Y.; Ren, H.; Liu, D.; Zhao, T.; Shi, X.; Cheng, H.; Zhao, N.; Li, Z.; Li, B.; Niu, H.; Zhuang, W.; Xie, J.; Chen, X.; Wu, J.; Ying, H.

Butanol recovery from acetone-butanol-ethanol (ABE) fed-batch fermentation using permeating-heating-gas was determined in this study. Fermentation was performed with Clostridium acetobutylicum B3 in a fibrous bed bioreactor and permeating-heating-gas stripping was used to eliminate substrate and product inhibition, which normally restrict ABE production and sugar utilization to below 20g/L and 60g/L, respectively. In batch fermentation (without permeating-heating-gas stripping), C. acetobutylicum B3 utilized 60g/L glucose and produced 19.9g/L ABE and 12g/L butanol, while in the integrated process 290g/L glucose was utilized and 106.27g/L ABE and 66.09g/L butanol were produced. The intermittent gas stripping process generated a highly concentrated condensate containing approximately 15% (w/v) butanol, 4% (w/v) acetone, a small amount of ethanol (<1%), and almost no acids, resulting in a highly concentrated butanol solution [~70% (w/v)] after phase separation. Butanol removal by permeating-heating-gas stripping has potential for commercial ABE production.

2014

Elsevier Applied Science

0960-8524

164

pp.276-284

10.1016/j.biortech.2014.04.107

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69315892

ABE fermentation; Permeating-heating-gas stripping; Fed-batch fermentation; Cell immobilization