초록
<P><B>Abstract</B></P> <P>Oil palm empty fruit bunch (EFB), a plentiful agricultural waste in Malaysia was evaluated for the production of succinic acid (SA) via simultaneous saccharification and fermentation (SSF) using <I>A. succinogenes</I> ATCC 55618. In the current study, EFB was pretreated using 2 different pretreatment methods; autoclave alkali (AA) and sequential dilute acid microwave alkali (DA-MWA). The pretreated EFBs were hydrolyzed enzymatically and they were also used as a substrate for SA production. During enzymatic hydrolysis different ratios of <I>cellulase</I> and <I>cellobiase</I> were used. Results revealed the best yield of glucose accumulation (31.4 g L<SUP>-1</SUP>) was obtained when the ratio of <I>cellulase</I> and <I>cellobiase</I> was kept to 7:1. Subsequent findings also showed maximum concentration, (33.4 g L<SUP>−1</SUP>), yield (30.47 g g<SUP>−1</SUP>substrate) and productivity 0.69 g L<SUP>−1</SUP> h<SUP>−1</SUP> of SA was achieved when using sequential DA-MWA pretreated EFB sample. The results suggested that agriculture waste EFB has the potential to be an alternative substrate for efficient and economic SA production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Succinic acid was produced via SSF using <I>A. succinogenes</I> ATCC 55618 from EFB. </LI> <LI> EFB was pretreated using autoclave alkali and sequential dilute acid MW alkali. </LI> <LI> Best glucose yield was achieved when <I>cellulase</I> and <I>cellobiase</I> ratio was 7:1. </LI> <LI> Sequential DA-MWA pretreated EFB gives maximum yield and productivity of SA. </LI> <LI> EFB has potential to be an alternative cheap substrate for efficient SA production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>