초록
Aim: Hydrogen production by biological means is more advantageous as it utilizes renewable sources and produces very low or no carbondioxide. Since, lignocellulosic feedstocks are cheaper source, the present study was aimed to isolate Pseudomonas stutzeri from soil sample at country bricks chamber furnace. Methods: Final confirmation of the strain was done by 16S rRNA sequencing. The GC content was calculated. The isolated strain was inoculated in hydrogen production medium containing sugarcane bagasse replacing glucose. After the incubation period of 16 days, the gas collected was studied for hydrogen production through gas chromatography technique. Results: According to sequencing and phylogenetic tree results, our isolate was found to be P. stutzeriKF532951. The GC content of our strain was above 53%. Our strain could produce up to 3.4 mol H<SUB>2</SUB>/mol substrate by water displacement method. Conclusion: On the basis of the above results, it is concluded that our strain is able to produce hydrogen biologically.