초록
<P><B>Abstract</B></P> <P>The effects of N/C, P/C and Fe/C ratios on dark fermentative hydrogen gas production from activated carbon treated WPT hydrolysate were investigated using Box–Behnken statistical experiment design. N/C, P/C and Fe/C ratios were chosen as independent variables while the H<SUB>2</SUB> yield and SHPR were set as the objective functions. H<SUB>2</SUB> yield and SHPR functions were described by two quadratic model functions. The addition of a proper amount of N, P and Fe to the fermentation media was found to be essential to enhance the H<SUB>2</SUB> production performance. Linear and interaction terms of N/C and Fe/C did have a significant effect on the H<SUB>2</SUB> yield in the model function. However, the SHPR was significantly affected by the linear and interaction terms of N/C and P/C. The most convenient N/C, P/C and Fe/C ratios resulting maximum H<SUB>2</SUB> yield (0.656 mol H<SUB>2</SUB>/mol glucose) and SHPR (241.64 mL H<SUB>2</SUB>/g biomass.h) were determined as 0.05, 0.09 and 0.003 (w/w), respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> WPT hydrolysate can be used for dark fermentative H2 production after AC treatment. </LI> <LI> 5-HMF was efficiently removed from the hydrolysate by AC treatment. </LI> <LI> Optimum C/N/P/Fe ratio resulting highest HY and SHPR was 100/5/9/0.3 </LI> <LI> Highest HY and SHPR were 0.525 mol H<SUB>2</SUB>/mol glucose and 226.64 mL H<SUB>2</SUB>/g biomass.h, respectively. </LI> </UL> </P>