초록
This study evaluates acid pretreated waste paper hydrolyzate as feedstock for bioethanol production. The presence of 70.12+/-4.88% carbohydrates (holocellulose) makes waste paper a prospective and renewable biomass for bioethanol production. The waste paper was found to contain α-cellulose (61.5+/-3.49%), pentosans (7.42+/-0.36%), lignin (16.33+/-0.96%), ash (12.50+/-0.33%) and moisture (8.28+/-0.63%). Conditions for the dilute acid pretreatment of waste paper were optimized by varying solid/liquid ratio 1:8-1:14 (w/v), reaction time 01-06h, and sulfuric acid concentration 0.005-1.00N at 120<SUP>o</SUP>C in an autoclave. The conditions optimized for acid hydrolysis of waste paper were 0.50N H<SUB>2</SUB>SO<SUB>4</SUB> at 120<SUP>o</SUP>C for 02h reaction time keeping biomass:acid ratio 1:10 (w/v) for recovery of reducing sugars from waste paper hydrolyzate. Fermentation of acid hydrolyzate of waste paper with Pichia stipitis under optimum condition resulted in ethanol production 3.73+/-0.16g/l with 77.54+/-4.47% of fermentation efficiency.