BioChem - DOS

Utilization of wheat for biohydrogen production by a combination of solid-state fermentation and batch fermentation

International journal of hydrogen energy

Han, W.; Liu, D.N.; Li, Y.F.; Zhao, H.T.; Ren, N.Q.

A novel biological strategy of utilizing wheat as nutrient source for hydrogen production was developed. Wheat bran was used in the solid-state fermentations of Aspergillus awamori and Aspergillus oryzae that produce amylolytic and proteolytic enzyme streams, respectively. The resulting streams were added to wheat flour to generate nutrients-rich (glucose and free amino nitrogen (FAN)) solutions. Both glucose and FAN increased with increasing of wheat flour mass ratio from 3% to 10% (w/v) and the highest glucose (43.92 g/L) and FAN (944.53 mg/L) were obtained at wheat flour mass ratio of 10%. The wheat flour hydrolyzates were then used as the feedstock for biohydrogen production by Biohydrogenbacterium R3. The highest cumulative hydrogen production of 4.52 L was happened at glucose concentration of 33.15 g/L. However, the maximum hydrogen yield (2.34 mol H<SUB>2</SUB>/mol glucose) and hydrogen production (0.074 L H<SUB>2</SUB>/g wheat flour) were obtained at glucose concentration of 16 g/L.

2015

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

40

17

pp.5849-5855

10.1016/j.ijhydene.2015.03.036

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72017175

Hydrogen production; Solid-state fermentation; Aspergillus awamori; Aspergillus oryzae; Biohydrogenbacterium R3