초록
The aftermath of mining weathered crust elution-deposited rare earth ore produces a large amount of residual ammonium leaching solution, which causes ammonia and nitrogen pollution to the mine site. Recently, denitrification by heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria has attracted much attention. However, limited studies exist regarding the denitrification process of HN-AD bacteria. In this study, we combined four strains of HN-AD bacteria, Pseudomonas fulva K3, Pseudomonas mosselii K17, Klebsiella oxytoca A12, and Enterobacter hormaechei A16, obtained from rare earth element leaching sites, to select the best microbial consortium for ammonia nitrogen removal. We designed an ammonia removal process applicable to HN-AD bacteria to directly remove ammonia nitrogen from acidic leaching solutions. The experimental results demonstrated that the most efficient microbial consortium for ammonia nitrogen removal to be K3 + K17 + A16, with a removal efficiency of 89.68% for 8 h. In this process, considering the influencing factors of the ammonia removal process, the larger the influent flow rate and influent ammonia nitrogen concentration, the greater the ammonia nitrogen accumulation and pH decrease in the reactor. In consecutive multi-batch experiments, the ammonia removal process was used to remove ammonia nitrogen, at concentrations of 100-600 mg/L, from the simulated leaching solution at pH 4-7, whereby the effluent ammonia nitrogen concentration was lower than 15 mg/L. The results demonstrate that the ammonia removal process is highly feasible and stable. These findings will provide new ideas for the application of HN-AD bacteria and new methods for the removal of ammonia nitrogen from acidic leaching solutions.