초록
<P>2-Butanone is an important commodity chemical of wide application in different areas. In this study, <I>Klebsiella pneumoniae</I> was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. By introducing the diol dehydratase from <I>Lactobacillus brevis</I> and deleting the <I>ldhA</I> gene encoding lactate dehydrogenase, the engineered <I>K</I>. <I>pneumoniae</I> was able to accumulate 246 mg/L of 2-butanone in shake flask. With further optimization of culture condition, the titer of 2-butanone was increased to 450 mg/L. This study lays the basis for developing an efficient biological process for 2-butanone production.</P>