BioChem - DOS

Enhanced extraction of reducing sugars from fruit of Hovenia dulcis with treatment of cellulase and sequential production of ethanol and acetic acid containing ampelopsin from extracted reducing sugars

Industrial crops and products

Park, Jin-Woo; Park, Kyung-Geun; Lee, Na-Yeon; Lee, Jae-Hong; Lee, Jin-Woo

<P><B>Abstract</B></P> <P>The treatment of the fruit of <I>Hovenia dulcis</I> with the cellulase produced by <I>Bacillus amyloliquefaciens</I> DL-3 for the extraction of reducing sugars increased the release of sugars and decreased the extraction temperature and time. The production of the reducing sugars with the treatment of cellulase was 1.43 times higher than that without cellulase. The cellulase produced by <I>B. amyloliquefaciens</I> DL-3 had three different types of cellulosic activities, which seemed to result in the enhanced extraction of reducing sugars. These reducing sugars were used as the substrate for the sequential production of ethanol and acetic acid. The production of ethanol by <I>Saccharomyces cerevisiae</I> KCCM 11293 from 36.1 g/L reducing sugars was 18.0 g/L for 72 h and the sequential production of acetic acid by <I>Acetobacter pasteurianus</I> KCCM 40117 from 18.0 g/L ethanol was 18.4 g/L for 72 h. The concentration of ampelopsin in the fermentation broth was maintained throughout the sequential productions of ethanol and acetic acid.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced extraction of reducing sugars from the fruit of <I>H. dulcis.</I> </LI> <LI> Statistical optimization of extraction conditions of reducing sugars with cellulose. </LI> <LI> Sequential production of ethanol and acetic acid from reducing sugars. </LI> <LI> Production of ethanol and acetic acid broths containing ampelopsin. </LI> <LI> Application of ethanol and acetic acid for functional beverage and vinegar. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2019

Elsevier

0926-6690

139

pp.111522

10.1016/j.indcrop.2019.111522

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97712758

Acetic acid; Cellulase; Ethanol; Extraction; Hoveniadulcis; Reducing sugars