BioChem - DOS

Succinic acid production by immobilized cultures using spent sulphite liquor as fermentation medium

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Alexandri, Maria; Papapostolou, Harris; Stragier, Lutgart; Verstraete, Willy; Papanikolaou, Seraphim; Koutinas, Apostolis A.

<P><B>Abstract</B></P> <P>Spent sulphite liquor (SSL) was used as carbon source for the production of succinic acid using immobilized cultures of <I>Actinobacillus succinogenes</I> and <I>Basfia succiniciproducens</I> on two different supports, delignified cellulosic material (DCM) and alginate beads. Fed-batch immobilized cultures with <I>A. succinogenes</I> in alginates resulted in higher sugar to succinic acid conversion yield (0.81g/g) than the respective yield achieved (0.65g/g) when DCM immobilized cultures were used. The final succinic acid concentration and yield achieved in fed-batch with immobilized cultures of <I>B. succiniciproducens</I> in alginates (45g/L and 0.66g/g) were higher than <I>A. succinogenes</I> immobilized cultures (35.4g/L and 0.61g/g) using nano-filtrated SSL as fermentation medium. Immobilized cultures of <I>B. succiniciproducens</I> in alginate beads were reused in four sequential fed-batch fermentations of nano-filtrated SSL leading to the production of 64.7g of succinic acid with a yield range of 0.42&ndash;0.67g/g and productivity range of 0.29&ndash;0.65g/L/h. The immobilized cultures improved the efficiency of succinic acid production as compared to free cell cultures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cell immobilization on DCM and alginate beads was investigated. </LI> <LI> Immobilized cells of <I>A. succinogenes</I> were able to ferment crude SSL. </LI> <LI> Ultra- and nano-filtration of SSL led to enhanced succinic acid production. </LI> <LI> <I>B. succiniciproducens</I> immobilized in alginates produced 45g/L of succinic acid. </LI> <LI> The biocatalyst was efficiently re-used in 4 sequential fed-batches. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2017

Elsevier

0960-8524

238

pp.214-222

10.1016/j.biortech.2017.03.132

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77891314

Succinic acid; Immobilization; Alginate beads; Delignified cellulosic material; Spent sulphite liquor