BioChem - DOS

Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content

Journal of biotechnology

Gouveia, Joao Diogo; Ruiz, Jesus; van den Broek, Lambertus A.M.; Hesselink, Thamara; Peters, Sander; Kleinegris, Dorinde M.M.; Smith, Alison G.; van der Veen, Douwe; Barbosa, Maria J.; Wijffels, Rene H.

<P><B>Abstract</B></P> <P> <I>Botryococcus braunii</I> can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of <I>Botryococcus braunii</I> but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 <I>B. braunii</I> strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8gL<SUP>&minus;1</SUP> day<SUP>&minus;1</SUP>, while hydrocarbon production ranged from none to up to 42% per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20% to 76% per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463mgL<SUP>&minus;1</SUP> in CCALA778.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 16 commercial available strains compared under the same conditions. </LI> <LI> High variability in carbohydrates and hydrocarbon content among the strains. </LI> <LI> Glucose and galactose are the main monomeric saccharides for all strains. </LI> <LI> Fucose highly present in CCALA778 strain. </LI> <LI> K1489 strain is characterized for the first time. </LI> </UL> </P>

2017

Elsevier

cc-by

0168-1656

1873-4863

248

pp.77-86

10.1016/j.jbiotec.2017.03.008

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77707415

Botryococcus braunii; Hydrocarbons; Carbohydrate; Galactose; Fucose; Microalgae