초록
<P><B>Abstract</B></P> <P>In this study, gene YALI0F01650g has been isolated and characterized. Several experimental evidences suggest that the identified gene, renamed <I>EYD1</I>, encodes an erythritol dehydrogenase. An efficient bioreactor process for the bioconversion of erythritol into erythrulose was also developed. Using constitutive expression of <I>EYD1</I> in a <I>Y. lipolytica</I> mutant containing a disrupted <I>EYK1</I> gene, which encodes erythrulose kinase, erythrulose could be synthesized from erythritol at a rate of 0.116g/g<SUB>DCW</SUB>.h and with a bioconversion yield of 0.64g/g.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Y. lipolytica</I> is able to bio-convert erythritol into erythrulose. </LI> <LI> Gene YALI0F01650g, renamed as <I>EYD1,</I> encodes an erythritol dehydrogenase. </LI> <LI> Overexpression of <I>EYD1</I> in a <I>Y. lipolytica</I> Δ<I>eyk1</I> mutant permits the bioconversion of erythritol into erythrulose. </LI> </UL> </P>