BioChem - DOS

Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars

Journal of industrial microbiology & biotechnology

Hughes, Stephen R.; Gibbons, William R.; Bang, Sookie S.; Pinkelman, Rebecca; Bischoff, Kenneth M.; Slininger, Patricia J.; Qureshi, Nasib; Kurtzman, Cletus P.; Liu, Siqing; Saha, Badal C.; Jackson, John S.; Cotta, Michael A.; Rich, Joseph O.; Javers, Jeremy E.

<P><I>Scheffersomyces</I> (formerly <I>Pichia</I>) <I>stipitis</I> NRRL Y-7124 was mutagenized using UV-C irradiation to produce yeast strains for anaerobic conversion of lignocellulosic sugars to ethanol. UV-C irradiation potentially produces large numbers of random mutations broadly and uniformly over the whole genome to generate unique strains. Wild-type cultures of <I>S</I>. <I>stipitis</I> NRRL Y-7124 were subjected to UV-C (234&nbsp;nm) irradiation targeted at approximately 40% cell survival. When surviving cells were selected in sufficient numbers via automated plating strategies and cultured anaerobically on xylose medium for 5&nbsp;months at 28°C, five novel mutagenized <I>S</I>. <I>stipitis</I> strains were obtained. Variable number tandem repeat analysis revealed that mutations had occurred in the genome, which may have produced genes that allowed the anaerobic utilization of xylose. The mutagenized strains were capable of growing anaerobically on xylose/glucose substrate with higher ethanol production during 250- to 500-h growth than a <I>Saccharomyces cerevisiae</I> yeast strain that is the standard for industrial fuel ethanol production. The <I>S. stipitis</I> strains resulting from this intense multigene mutagenesis strategy have potential application in industrial fuel ethanol production from lignocellulosic hydrolysates.</P>

2012

Springer-Verlag

1367-5435

1476-5535

39

1

pp.163-173

10.1007/s10295-011-1012-x

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58270871

Pichia stipitis [Scheffersomyces stipitis]; Cellulosic fuel ethanol; Anaerobic xylose fermentation; Yeast UV mutagenesis