BioChem - DOS

Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3 propanediol and butyric acid

Biochemical engineering journal

Varrone, Cristiano; Floriotis, Georgis; Heggeset, Tonje M.B.; Le, Simone B.; Markussen, Sidsel; Skiadas, Ioannis V.; Gavala, Hariklia N.

<P><B>Abstract</B></P> <P>This study investigated the performance of different mixed microbial cultures (MMC) able to ferment crude glycerol generated from animal fat-based biodiesel to produce 1,3 propanediol (1,3 PDO) and butyric acid, under non-sterile conditions. Eight different continuous flow stirred-tank reactors (CSTR) were set up with different inoculum types and growth media. The distribution of metabolic products under variable operating conditions was determined. All MMC were characterized from a kinetic point of view and overall stoichiometric reactions were constructed. Changes in the microbial communities were monitored by means of Next Generation Sequencing (NGS). Maximum substrate degradation rate reached approximately 110g/L/d of glycerol (with a productivity of 38g/L/d and 11g/L/d for 1,3 PDO and butyric acid, respectively), obtained with an hydraulic retention time of 12h and 60g/L feed. The maximum feed concentration reached almost 90g/L, leading though to an incomplete substrate degradation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Stable and efficient (99%) conversion of crude glycerol in CSTR by MM. </LI> <LI> Maximum productivity of 37.8g/L/d 1,3 PDO and 11.14g/L/d butyric acid. </LI> <LI> Selected MMC able to grow on 90g/L of animal fat derived crude glycerol. </LI> <LI> Maximum glycerol conversion rate of 110g/L/d. </LI> </UL> </P>

2017

Elsevier

1369-703x

128

pp.149-161

10.1016/j.bej.2017.09.012

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79614394

https://backend.orbit.dtu.dk/ws/files/138399122/MARAC_1_s2.0_S1369703X17302395_main.pdf

Crude glycerol; CSTR; Fermentation; Mixed microbial cultures; 1,3 propanediol; Butyric acid