초록
Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B<SUB>12</SUB>-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B<SUB>12</SUB>, suggesting that the intrinsic pseudovitamin B<SUB>12</SUB> served as a substitute of coenzyme B<SUB>12</SUB>. The highest titers of 1,3-PDO (3.79+/-0.23mM; 288+/-17.7mg/L) and glycerol (12.62+/-1.55mM; 1.16+/-0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.