초록
<P><B>Abstract</B></P><P>Production of reducing sugar by hydrolysis of corncob material with <I>Streptomyces</I> sp. cellulase and ethanol fermentation of cellulosic hydrolysate was investigated. Cultures of <I>Streptomyces</I> sp. T3-1 improved reducing sugar yields with the production of CMCase, Avicelase and β-glucosidase activity of 3.8, 3.9 and 3.8 IU/ml, respectively. CMCase, Avicelase, and β-glucosidase produced by the <I>Streptomyces</I> sp. T3-1 favored the conversion of cellulose to glucose. It was recognized that the synergistic interaction of endoglucanase, exoglucanase and β-glucosidase resulted in efficient hydrolysis of cellulosic substrate. After 5 d of incubation, the overall reducing sugar yield reached 53.1 g/100 g dried substrate. Further fermentation of cellulosic hydrolysate containing 40.5 g/l glucose was performed using <I>Saccharomyces cerevisiae</I> BCRC 21812, 14.6 g/l biomass and 24.6 g/l ethanol was obtained within 3 d. The results have significant implications and future applications regarding to production of fuel ethanol from agricultural cellulosic waste.</P> <P><B>Highlights</B></P><P>► Hydrolysis efficiency of cellulosic material was improved by <I>Streptomyces</I> sp. T3-1. ► The synergistic interaction of cellulases resulted in efficient hydrolysis of substrate. ► A significantly higher ethanol yield (0.67 g ethanol/g glucose) was achieved.</P>