BioChem - DOS

Efficient production of pullulan using rice hull hydrolysate by adaptive laboratory evolution of Aureobasidium pullulans

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Wang, D.; Ju, X.; Zhou, D.; Wei, G.

Pullulan production by Aureobasidium pullulans CCTCC M 2012259 using rice hull hydrolysate as the carbon source was conducted. The acetic acid in the hydrolysate was demonstrated to exert a negative effect on pullulan biosynthesis. Instead of employing expensive methods to remove acetic acid from the hydrolysate, a mutant A. pullulans ARH-1 was isolated following 20 cycles of adaptive laboratory evolution of the parental strain on medium containing acetic acid. The maximum pullulan production achieved by the adapted mutant at 48h using the hydrolysate of untreated rice hull was 22.2gL<SUP>-1</SUP>, while that obtained by the parental strain at 60h was 15.6gL<SUP>-1</SUP>. The assay of key enzymes associated with pullulan biosynthesis revealed that acetic acid inhibited enzyme activity rather than suppressing enzyme synthesis. These results demonstrated that adaptive evolution highly improved the efficiency of pullulan production by A. pullulans using the hydrolysate of untreated rice hull.

2014

Elsevier Applied Science

0960-8524

164

pp.12-19

10.1016/j.biortech.2014.04.036

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69315913

Pullulan; Aureobasidium pullulans; Rice hull hydrolysate; Acetic acid; Adaptive laboratory evolution