Production of xylitol by expressing xylitol dehydrogenase and alcohol dehydrogenase from Gluconobacter thailandicus and co-biotransformation of whole cells
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Production of xylitol by expressing xylitol dehydrogenase and alcohol dehydrogenase from Gluconobacter thailandicus and co-biotransformation of whole cells
<P><B>Abstract</B></P> <P>In the present study, recombinant strains were constructed for xylitol production by cloning and expressing the novel xylitol dehydrogenase (<I>xdh</I>) and alcohol dehydrogenase (<I>adh</I>) genes in <I>E. coli</I> BL21 (DE3) from <I>Gluconobacter thailandicus</I> CGMCC1.3748. The optimum pH, temperature, specific activity and kinetic parameters were further investigated for purified XDH. The co-culture of <I>G. thailandicus</I> (30 g/L), BL21-<I>xdh</I> (20 g/L) and BL21-<I>adh</I> (20 g/L) produced 34.34 g/L of xylitol after 48 h in the presence of 40 g/L <SMALL>D</SMALL>-arabitol and 2% ethanol. The concentration of xylitol produced in this co-biotransformation was found to be 2.7-folds higher than the xylitol yield of <I>G. thailandicus</I> alone, while the yield was increased by 4.8% when compared to that of <I>G. thailandicus</I> mixed with BL21-<I>xdh</I> under the similar experimental conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel <I>xdh</I> and <I>adh</I> were cloned from <I>Gluconobacter thailandicus</I> CGMCC1.3748. </LI> <LI> Two recombinant <I>E. coli</I> strains were constructed by expressing <I>xdh</I> and <I>adh</I> individually. </LI> <LI> Whole cell biotransformation was done using both recombinant and original strains. </LI> <LI> The mixed culture of recombinant and original strains produced 2.7-folds higher xylitol. </LI> </UL> </P>