BioChem - DOS

Alkane production from fatty alcohols by the combined reactions catalyzed by an alcohol dehydrogenase and an aldehyde-deformylating oxygenase

Bioscience, biotechnology, and biochemistry

Sui, Yu-An; Maruyama, Satoshi; Okada, Natsumi; Ito, Masakazu; Muramatsu, Masayoshi; Obata, Shusei; Ogawa, Jun; Kishino, Shigenobu

<P><B>ABSTRACT</B><P>PsADH, an alcohol dehydrogenase originating in Pantoea sp. was characterized and found to convert a broad variety of fatty alcohols into their corresponding aldehydes, the substrates of alkane biosynthesis. By coupling PsADH with NpAD, a cyanobacterial aldehyde-deformylating oxygenase, and by optimizing the conditions of the enzyme-catalyzed reactions, we achieved a 52% conversion of 1-tetradecanol to tridecane. We further applied this system to generate alkanes ranging from C5-17. These alkanes can be used as biofuels, suggesting that introducing a suitable alcohol dehydrogenase is an effective strategy to utilize fatty alcohols for alkane production.</P></P>

2023

Oxford University Press

0916-8451

1347-6947

87

8

pp.925-932

10.1093/bbb/zbad056

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART125768359