BioChem - DOS

Ethanol production from wheat straw by recombinant Escherichia coli strain FBR5 at high solid loading

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Saha, Badal C.; Nichols, Nancy N.; Cotta, Michael A.

<P><B>Highlights</B></P><P>&#x025BA; Yield of total sugars from wheat straw (150g/L) is 86g/L. &#x025BA; Recombinant <I>Escherichia coli</I> FBR5 produces ethanol at pH7.0 from un-detoxified wheat straw. &#x025BA; SSF offers distinct advantage over SHF (reduces total time and enzyme dose). &#x025BA; First report of above 4% ethanol production from lignocellulose by the strain.</P> <P><B>Abstract</B></P><P>Ethanol production by a recombinant bacterium from wheat straw (WS) at high solid loading by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) was studied. The yield of total sugars from dilute acid pretreated WS (150g/L) after enzymatic saccharification was 86.3&plusmn;1.5g/L. The pretreated WS was bio-abated by growing a fungal strain aerobically in the liquid portion for 16h. The recombinant <I>Escherichia coli</I> strain FBR5 produced 41.1&plusmn;1.1gethanol/L from non-abated WS hydrolyzate (total sugars, 86.6&plusmn;0.3g/L) in 168h at pH7.0 and 35&deg;C. The bacterium produced 41.8&plusmn;0.0gethanol/L in 120h from the bioabated WS by SHF. It produced 41.6&plusmn;0.7gethanol/L in 120h from bioabated WS by fed-batch SSF. This is the first report of the production of above 4% ethanol from a lignocellulosic hydrolyzate by the recombinant bacterium.</P>

2011

0960-8524

102

23

pp.10892-10897

10.1016/j.biortech.2011.09.041

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57757422

Ethanol; Recombinant ethanologenic Escherichia coli; Separate hydrolysis and fermentation; Simultaneous saccharification and fermentation; Wheat straw hydrolyzate