BioChem - DOS

The isolation, characterization and effect of lignin isolated from steam pretreated Douglas-fir on the enzymatic hydrolysis of cellulose

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Nakagame, S.; Chandra, R.P.; Kadla, J.F.; Saddler, J.N.

Douglas-fir was SO<SUB>2</SUB>-steam pretreated at different severities (190, 200, and 210<SUP>o</SUP>C) to assess the possible negative effect of the residual and isolated lignins on the enzymatic hydrolysis of the steam pretreated substrates. When various isolated lignins were added to the Avicel hydrolysis reactions, the decrease in glucose yields ranged from 15.2% to 29.0% after 72h. It was apparent that the better hydrolysis yields obtained at higher pretreatment severities were more a result of the greater accessibly of the cellulose rather than any specific change in the non-productive binding of the lignin to the enzymes. FTIR and <SUP>13</SUP>C NMR characterization indicated that the lignin in the steam pretreated substrates became more condensed with increasing severity, suggesting that the cellulases were adsorbed to the lignin by hydrophobic interactions. Electrostatic interactions were also involved as the positively charged cellulase components were preferentially adsorbed to the lignins.

2011

Elsevier Applied Science

0960-8524

102

6

pp.4507-4517

10.1016/j.biortech.2010.12.082

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55435312

Cellulose-lignin interaction; Lignocellulose; Lignin; SO2-catalyzed steam pretreatment; Cellulases