초록
<P><B>Abstract</B></P> <P>In this study, crude glycerol was demonstrated as feedstock to use in the fed-batch fermentation of 1,3-propanediol production by <I>Klebsiella pneumoniae</I> ATCC 8724. To increase the yield and productivity of 1,3-propanediol, several factors for fed-batch fermentation were investigated, which included: initial substrate conditions, feeding strategy and fermentation conditions. Both performances of 1,3-propandiol and by-products were observed and studied for improved fed-batch fermentation. Increasing of initial working volume, continuous feeding, and culture pH switch strategy showed positive performance for this fed-batch process. The maximum 1,3-propandiol concentration, yield, and productivity were achieved 62.72 g/l, 0.73 mol/mol and 1.74 g/l/h, respectively. With the ignorable inhibitory effect, a large quantity of crude glycerol from industrial biodiesel process would be an attractive feedstock for value-added chemical, 1,3-propandiol, manufacture. The improvement of fed-batch fermentation could also be achieved by strategy optimization in further applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The concentration and yield of 1,3-PDO were 62.72 g/l and 0.73 mol/mol, respectively. </LI> <LI> The 1,3-PDO productivity was 1.74 g/l/h by a continuous feeding strategy within 36 h. </LI> <LI> By-products generation was reduced by a culture strategy of switching pH control. </LI> </UL> </P>