BioChem - DOS

Enhanced sugar production from pretreated barley straw by additive xylanase and surfactants in enzymatic hydrolysis for acetone-butanol-ethanol fermentation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Yang, Ming; Zhang, Junhua; Kuittinen, Suvi; Vepsä lä inen, Jouko; Soininen, Pasi; Keinä nen, Markku; Pappinen, Ari

<P><B>Abstract</B></P> <P>This study aims to improve enzymatic sugar production from dilute sulfuric acid-pretreated barley straw for acetone&ndash;butanol&ndash;ethanol (ABE) fermentation. The effects of additive xylanase and surfactants (polyethylene glycol [PEG] and Tween) in an enzymatic reaction system on straw hydrolysis yields were investigated. By combined application of 2g/100g dry-matter (DM) xylanase and PEG 4000, the glucose yield was increased from 53.2% to 86.9% and the xylose yield was increased from 36.2% to 70.2%, which were considerably higher than results obtained with xylanase or surfactant alone. The ABE fermentation of enzymatic hydrolysate produced 10.8g/L ABE, in which 7.9g/L was butanol. The enhanced sugar production increased the ABE yield from 93.8 to 135.0g/kg pretreated straw. The combined application of xylanase and surfactants has a large potential to improve sugar production from barley straw pretreated with a mild acid and that the hydrolysate showed good fermentability in ABE production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effects of additive xylanase and surfactants on hydrolysis of barley straw were studied. </LI> <LI> High sugar yields were obtained by combined application of xylanase and PEG 4000. </LI> <LI> Surfactants contributed to the synergistic cooperation of xylanase with cellulase. </LI> <LI> The hydrolysate of barley straw was efficiently utilized in biobutanol production. </LI> <LI> The enhanced sugar production increased the ABE production yield. </LI> </UL> </P>

2015

Elsevier

0960-8524

189

pp.131-137

10.1016/j.biortech.2015.04.008

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72052679

Barley straw; Enzymatic hydrolysis; Xylanase; Surfactants; Acetone&#x2013; butanol&#x2013; ethanol;