초록
<P><B>Abstract</B></P> <P>Succinic acid has attracted interest worldwide as a precursor of many industrially crucial chemicals. Biosynthesis of succinic acid from biomass is developing as an environmentally friendly strategy now. Conversion of sweet potato waste (SPW) to succinic acid could implement high-value utilization of biomass, cut cost of the fermentation process and reduce the pollution of environment. Engineered <I>Escherichia coli</I> (<I>E. coli</I>) strain HD134 under the control of anaerobically-induced <I>nirB</I> promoter from <I>Salmonella enterica</I> (P<SUB>SnirB</SUB>) could produce about 16.30 g/L succinic acid with a yield of 0.83 g/g after 48 h on glucose. With SPW hydrolysate as the substrate, 18.65 g/L succinic acid with a yield of 0.94 g/g after 48 h fermentation achieved. Compared to SD134 under <I>Trc</I> control induced with Isopropyl β-D-Thiogalactoside (IPTG), this concentration and yield represented an 8.56% and 6.82% increase, respectively. The use of anaerobically-induced P<SUB>SnirB</SUB> not only could attain higher production of succinic acid than IPTG-induced <I>Trc</I> promoter, but omit cost of expensive exogenous inducers. The efficient production of succinic acid from SPW was firstly studied by anaerobically-induced P<SUB>SnirB</SUB> control, which achieved relative lower cost compared to glucose as substrate and IPTG as the inducer. This novel fermentation process conduces to the cosmically industrial succinic acid bioproduction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Anaerobically-induced P<SUB>SnirB</SUB> was firstly explored for the succinic acid production. </LI> <LI> The use of anaerobically-induced P<SUB>SnirB</SUB> could omit cost of exogenous inducers. </LI> <LI> SPW were explored for succinic acid production. </LI> <LI> 18.65 g/L succinic acid from SPW hydrolysate was produced. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>The overall strategy for succinic acid production. 1 glucose phosphotransferase; 2 carbonic anhydrase; 3 phosphoenolpyruvate carboxylase; 4 malate dehydrogenase; 5 fumarase; 6 fumarate reductase.</P> <P>[DISPLAY OMISSION]</P>