BioChem - DOS

Biohydrogen production from pure and natural lignocellulosic feedstock with chemical pretreatment and bacterial hydrolysis

International journal of hydrogen energy

Lo, Y.C.; Su, Y.C.; Cheng, C.L.; Chang, J.S.

Pretreatment and saccharification of lignocellulosic materials is the key technology affecting the efficiency of cellulosic biohydrogen production. In this work, two pure cellulosic materials (i.e., carboxymethyl-cellulose (CMC) and xylan) were directly hydrolyzed (without pretreatment) by a cellulolytic isolate Cellulomonas uda E3-01 able to release extracellular cellulolytic enzymes. Natural cellulosic feedstock (i.e., sugarcane bagasse) was chemically pretreated prior to the bacterial hydrolysis.A temperature-shift strategy (35 <SUP>o</SUP>C for cellulolytic enzymes production and 45 <SUP>o</SUP>C for hydrolysis reaction) was used to increase the production of reducing sugars during the bacterial hydrolysis. The hydrolysates of CMC, xylan, and bagasse were efficiently converted to H<SUB>2</SUB> via dark fermentation with Clostridium butyricum CGS5. The maximum hydrogen yield was 8.80 mmol H<SUB>2</SUB>/g reducing sugar (i.e., 1.58 mol H<SUB>2</SUB>/mol hexose) for CMC, 6.03 mmol H<SUB>2</SUB>/g reducing sugar (i.e., 0.91 mol H<SUB>2</SUB>/mol pentose) for xylan, and 6.01 mmol H<SUB>2</SUB>/g reducing sugar for bagasse.

2011

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

36

21

pp.13955-13963

10.1016/j.ijhydene.2011.03.100

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57433406

Biohydrogen; Cellulose hydrolysis; Cellulase; Xylanase; Cellulomonas uda; Clostridium butyricum