초록
Efficient H<SUB>2</SUB> producing bacterial strain Thermoanaerobacterium thermosaccharolyticum IIT BT-ST1 was isolated from the anaerobic digester. Taguchi design of experiment was applied to evaluate the influence of the temperature, pH, glucose, FeSO<SUB>4</SUB> and yeast extract on H<SUB>2</SUB> production with three levels of orthogonal array in the experimental design. Temperature showed most significant influence on the H<SUB>2</SUB> production process. Investigation of mutual interaction between the process parameters was studied employing Box-Behnken design. Experimentally optimized process parameters (60 <SUP>o</SUP>C, pH 6.5, 20 mM FeSO<SUB>4</SUB>, 4 g L<SUP>-1</SUP> yeast extract and 12 g L<SUP>-1</SUP> glucose) gave the maximum H<SUB>2</SUB> production of 3930 mL L<SUP>-1</SUP> in 24 h, which have close resemblance with the theoretical values. Continuous H<SUB>2</SUB> production using packed bed reactor was studied. Maximum H<SUB>2</SUB> production rate of 1691 mL L<SUP>-1</SUP> h<SUP>-1</SUP> at a dilution rate of 0.6 h<SUP>-1</SUP> was observed which is about 10 times higher than the batch process.